Innovative platform streamlines rare single-cell enrichment, characterization and retrieval

Celsee Diagnostics, an innovator of cutting-edge microfluidic products for rare cell capture, characterization and retrieval, announced today that they are demonstrating their latest innovation, Celsee PREP SingleCell at the AACR Annual Meeting 2017.

Single-cell analysis is used in a vast array of life science disciplines and enables drilldown studies on individual cells to examine their heterogeneity. To date, cost of instrumentation, workflow complexity, capture efficiency and retrieval of viable cells has kept the technique from going mainstream. The simplified Celsee PREP SingleCell platform is designed as an economical, efficient and streamlined method for capturing and retrieving viable single cells that virtually any laboratory can adopt.

The patented microfluidic chip contains hundreds of thousands of nanowells. The workflow starts by pipetting a small volume of a heterogeneous cell suspension over the nanowells in the chip. The solution spreads, depositing cells into their own individual wells. Since deposited cells are trapped in their own individual nanowell, select cells can then be retrieved from the device using the Celsee SingleCell Retriever or other cellular micromanipulator.

“We have streamlined the workflow allowing cell capture, staining and pickup from the same slide. This reduces cell loss and has been shown to have almost 100 percent capture rate in spiked-cell experiments,” said Kalyan Handique, President and CEO of Celsee Diagnostics.

Information can be gathered on individual cells’ morphology or biomarker content by performing immunochemistry, DNA FISH, mRNA Fish or live cell staining directly on-chip, or single cells can be retrieved for downstream processing such as NGS, PCR, culture or cryopreservation.

About Celsee Diagnostics
Celsee Diagnostics develops, manufactures and sells innovative, integrated systems for rare and single cell analysis, enumeration and isolation for Oncology/Immuno-Oncology research and clinical research applications. The label-free Celsee PREP platforms perform all the required processing steps to isolate and execute downstream single-cell analysis including immunochemistry, DNA FISH and mRNA FISH, while the Celsee ANALYZER scans and analyzes the prepared slide automatically. The Celsee SingleCell system integrates isolation, staining and retrieval providing an unprecedented yield, almost 100%, of viable cells for culture, PCR or NGS, at a cost of 1/10 that of competitive systems.