With a new identity, liquid biopsy firm Celsee looks to move CTC isolation tech to clinic
NEW YORK (GenomeWeb) – Having recently changed its name and added new executives to its team, Plymouth, Michigan-based liquid biopsy firm Celsee Diagnostics is now focusing on growing its commercial operations around its circulating tumor cell enrichment products. fundas samsung galaxy s6 fundas huawei mate 20 lite fundas iphone xr fundas samsung galaxy a50 fundas huawei p8 fundas huawei p30 lite The firm, formerly known as DeNovo Sciences, is also making progress on a number of industry and academic collaborations, and is in the Institutional Review Board stages of a clinical trial with the ultimate goal of obtaining regulatory approval in Europe and the US to use its CTC isolation products in a clinical capacity, Grant Howes, Celsee’s new vice president of commercial operations, told GenomeWeb. fundas samsung galaxy j3 fundas samsung galaxy note10 fundas samsung galaxy s10 plus fundas samsung galaxy a3 fundas huawei p9 fundas samsung galaxy s5 fundas samsung galaxy s10e fundas huawei mate 10 fundas samsung galaxy s10 plus “The technology focuses around microfluidic slides that Celsee has developed,” he explained. fundas samsung galaxy s7 fundas samsung galaxy s6 fundas iphone custodia cover iphone fundas huawei p smart 2019 fundas samsung galaxy j5 fundas huawei mate 20 The slides were deliberately designed to be similar to standard microscope slides, to allow customers to perform microscopy directly on the enriched CTCs, without needing to transfer them. fundas samsung galaxy s7 edge fundas huawei mate 20 lite fundas samsung galaxy s9 fundas samsung galaxy a3 fundas samsung galaxy note9 fundas huawei p8 lite 2017 fundas iphone 6 6s fundas huawei p8 lite 2017 “It saves time, and, because every time you’re manipulating cells you’re going to lose some of them … it also gives you more accurate enumeration,” Howes said. fundas samsung galaxy s8 fundas samsung galaxy s20 ultra fundas huawei p smart 2019 fundas samsung galaxy a10 fundas huawei y5 2018 custodia cover huawei The core technology is filter-based and works with or without pre-fixation, with optional staining to further refine CTC purification. fundas samsung galaxy note5 fundas huawei p30 pro fundas iphone 6 6s fundas samsung galaxy a3 fundas samsung galaxy note10 plus Whole blood is diluted and passed through the microfluidic slide, within which are more than 52,000 wells that each have an eight-micron pore. fundas iphone 11 fundas huawei p9 lite fundas huawei y6 2018 “As the blood flows through, the leukocytes and red cells can get through the pores with no problem whatsoever, but circulating tumor cells are typically larger, and they will get trapped in the wells,” Howes explained. fundas huawei y6 2019 fundas samsung galaxy s10 plus fundas samsung galaxy s20 ultra fundas samsung galaxy a50 fundas samsung galaxy s10 The option for staining would include cytokeratin, which can be used to mark cells from the vast majority of solid tumors, Howes said. fundas huawei p20 fundas samsung galaxy s20 plus fundas samsung galaxy a50 coque iphone xr fundas huawei p30 fundas samsung galaxy s20 custodia iphone custodia cover iphone A stain for CD45 can be used “to exclude [from analysis] any residual leukocytes that might be hanging around.” The firm offers a manual, entry-level system called the Celsee Prep 100, that uses syringe-based force to push sample and stain through. fundas samsung galaxy note10 fundas iphone 11 pro fundas samsung galaxy a50 fundas samsung galaxy a50 custodia cover iphone coque iphone 7 That system can process a single sample at a time, while the automated Celsee Prep 400 processes four samples in parallel. fundas samsung galaxy a70 fundas samsung galaxy note8 fundas huawei p8 lite fundas huawei y6 2018 The Celsee Analyzer, meanwhile, functions with either Prep system. fundas samsung galaxy s6 edge plus fundas huawei p10 lite fundas iphone x xs custodia samsung fundas iphone 11 fundas samsung galaxy j3 It is a six-color fluorescent scanning microscope and software that can automatically provide enumeration, as well as create and store multiple images of the CTCs along with their locations within the microfluidic, Howes said. fundas iphone xs max fundas samsung galaxy j3 custodia cover huawei fundas samsung galaxy s10 fundas huawei p20 pro There is also a protocol to remove the CTCs from the slide for downstream processing using PCR or sequencing. fundas samsung galaxy s10 A recent PLoS One study demonstrated the system’s utility for molecular analysis using DNA and mRNA fluorescence in-situ hybridization (FISH). fundas samsung galaxy s9 plus fundas huawei p10 lite fundas huawei p smart 2019 fundas samsung galaxy a3 fundas iphone xr fundas huawei p30 pro That work was done in collaboration with researchers at Johns Hopkins, Northwestern, and Thomas Jefferson Universities, as well as the University of Michigan and two universities in Rome, Italy. fundas huawei p30 lite fundas samsung galaxy s8 plus fundas huawei p20 fundas iphone 11 pro max It demonstrated the system was able to purify CTCs from blood samples of 96 metastatic breast cancer patients, as well as 27 prostate and five colorectal cancer patients. fundas huawei p smart 2019 custodia cover huawei fundas iphone 6 6s plus fundas huawei mate 10 fundas huawei p9 fundas samsung galaxy s8 plus fundas samsung galaxy note9 It further showed, in a subset of samples from patients with metastatic prostate cancer, that CTC counts were significantly higher using the Celsee system versus the US Food and Drug Administration-approved CellSearch system from Veridex, although the authors acknowledge the small sample size. fundas iphone xr fundas huawei p smart fundas samsung galaxy note5 fundas samsung galaxy note9 custodia iphone fundas samsung galaxy note5 fundas huawei p8 “A key feature of this platform is the ability to integrate the process of sample preparation, image analysis of CTCs, and molecular characterization of the captured cells,” the researchers noted, adding that ongoing work now focuses on “recovering single cells from the microfluidic chip for PCR and next-generation sequencing analyses.” The platforms are research-use-only, not diagnostic tests at the moment, Howes pointed out. fundas samsung galaxy note5 custodia samsung fundas samsung galaxy s10 fundas samsung galaxy note5 fundas samsung galaxy a3 fundas huawei p9 lite fundas huawei p8 lite fundas huawei p9 However, Celsee is now beginning beta clinical trials and ultimately intends to seek CE-IVD and 510(k) clearance for the system. coque samsung a70 fundas huawei p10 Concurrent with its name changes last year, Celsee also brought in new executives to help propel the technology further. fundas samsung galaxy s20 ultra fundas samsung galaxy a40 custodia cover fundas samsung galaxy note10 Howes came aboard in early January of this year. fundas samsung galaxy j3 fundas samsung galaxy s10 fundas samsung galaxy note10 He was most recently director of marketing at Becton Dickinson’s BD Biosciences. fundas samsung galaxy note10 plus fundas huawei p8 Prior to that he spent about four years as vice president of marketing at Accuri Cytometers, which was purchased by Becton Dickinson in 2011 for $205 million. fundas samsung galaxy note10 fundas samsung galaxy s10 plus fundas samsung galaxy s4 fundas huawei mate 10 lite fundas huawei p20 pro He also had a 23-year tenure at Beckman Coulter where he was ultimately director of strategic marketing. bracelet perle fundas iphone fundas huawei p8 lite 2017 fundas iphone fundas huawei p8 lite 2017 fundas samsung galaxy s7 edge “My background is really cell-based assays,” Howes said, adding that he’s been able to apply that as along with his previous sales and marketing experience to jumpstart Celsee’s commercial operation. fundas iphone 11 pro fundas samsung galaxy a3 “We’re getting too many requests for demonstrations for the existing team here to manage,” he added. fundas samsung galaxy s10 plus fundas samsung galaxy a3 fundas samsung galaxy note8 fundas samsung galaxy note10 fundas huawei p20 fundas samsung galaxy s20 plus fundas samsung galaxy a5 coque iphone 8 fundas samsung galaxy a5 The company’s CEO is Kalyan Handique. fundas huawei y5 2018 fundas huawei p20 lite fundas samsung galaxy s9 plus fundas samsung galaxy s10e coque iphone 7 fundas huawei p30 pro fundas huawei y6 2019 fundas samsung galaxy a3 He was formerly co-founder and CTO of Handylab before that firm was acquired by Becton Dickinson in 2009 and the HandyLab Jaguar system was developed into the BD Max real-time PCR molecular diagnostics system. fundas huawei p smart fundas samsung galaxy s10e fundas samsung galaxy s7 Handique was then vice president of R&D Systems Development for BD Infectious Disease for about two years. fundas huawei p9 lite bague homme fundas samsung galaxy s8 plus fundas samsung galaxy s10 plus fundas samsung galaxy s20 Celsee is currently collaborating with recent BioMérieux spinout BioTheranostics, and will be presenting jointly at the American Association for Cancer Research annual meeting in April, “showing feasibility data for the use of BioTheranostics and Celsee technologies in tandem,” Howes said. custodia cover iphone fundas samsung galaxy note5 fundas iphone 11 fundas huawei p20 pro The firm has also submitted an abstract on analysis of CTCs after enrichment on its systems in collaboration with TJU, he said, and last year began a collaboration with South Texas Accelerated Research Therapeutics to perform whole-genome sequencing on circulating tumor cells. fundas iphone 11 fundas samsung galaxy s4 fundas samsung galaxy s6 fundas huawei mate 20 lite The firm is now focused on growing its current business by developing its customer and reference base. fundas huawei y6 2019 fundas samsung galaxy s7 fundas iphone 7 8 se2020 However, “If the technology can be used by other companies, we’re interested in a collaboration — we’re actually starting a couple of collaborations fairly soon with a view to see if our products could be incorporated into other people’s products,” Howes said. fundas iphone 7 8 plus fundas huawei p10 Media Contact: Nicole Litchfield Bioscribe, Inc.